Comparison of Automated (ISED) ESR Estimation with Conventional (WESTERGREN) Method for Validation of Automated Results

Background: ESR inspite of being considered as a non-specific heamatological test is widely used for the detection autoimmune diseases, infections and cancers. Although conventional gold standard methods are accurate authentic but having few biohazard risks more time consuming so thought to be replaced by automated which less not much expensive need validated comparing them with manual (Westergren) method. Objective: To compare values method i.e. Westergren estimation ISED (ALCOR) validate results obtained Study design: A Prospective cross-sectional study duration settings: was conducted at Pathology Department Pak Emirates Military Hospital Rawalpindi from November 2021 April 2022. Material methods: All samples were processed within 1 hour venipuncture. Hemolyzed discarded. separate EDTA sample tubes taken 3ml venous blood collected in each tube respectively. Results: 200 total Westergren's (Manual Method) ISED's estimates (Automated Method). Mean age patients 50.74 ± 16.84 years range 8 88 years, out 54 % male 46 females, 51 OPD 49 admitted Conclusion: The Gold has proven efficient CV p-value calculated individually well all cases statistically insignificant > 0.05%. Keywords: Automated method, Erythrocyte Sedimentation Rate,